Updated: Aug 21, 2026
No. of Questions: 562 Questions & Answers with Testing Engine
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| Certification Vendor: | American Society for Clinical Pathology (ASCP) |
| Exam Name: | MLT(ASCP) Certification Examination |
| Exam Number: | MLT(ASCP) |
| Exam Duration: | 150 minutes |
| Exam Price: | Approximately USD 200–240 (varies by eligibility route and region) |
| Real Exam Qty: | 100 multiple-choice questions |
| Available Languages: | English |
| Certificate Validity Period: | 3 years (ASCP certification maintenance required via continuing education) |
| Passing Score: | Scaled score of 400 |
| Related Certifications: | MLS(ASCP) M(ASCP)CM HT(ASCP) PBT(ASCP) |
| Exam Format: | Multiple-choice, Computer-based testing, Single-best answer |
| Recommended Training: | ASCP BOC Study Materials and Exam Content Guidelines |
| Exam Registration: | ASCP Board of Certification (BOC) application portal |
| Sample Questions: | ASCP ASCP-MLT Sample Questions |
| Exam Way: | Computer-based exam administered at Pearson VUE testing centers |
| Pre Condition: | Eligibility requires completion of an accredited MLT program or equivalent education/clinical experience as defined by ASCP BOC routes of qualification. |
| Official Syllabus URL: | https://www.ascp.org/content/board-of-certification/get-credentialed |
| Section | Objectives |
|---|---|
| Microbiology | - Clinical microbiological techniques
|
| Urinalysis and Body Fluids | - Fluid analysis and interpretation
|
| Clinical Chemistry | - Metabolic and analytical testing
|
| Hematology | - Blood cell analysis and disorders
|
| Laboratory Operations | - Quality and safety in the clinical lab
|
| Blood Banking / Immunohematology | - Blood group systems and antibody identification
|
1. Waived tests are those considered to have an insignificant risk of erroneous results. Which of the following is NOT an example of a waived test?
A) fructosamine
B) potassium
C) blood glucose by home meters
D) spun hematocrit
2. A positive spot test shows agglutination of horse erythrocytes when added to patient serum previously absorbed with guinea pig kidney, but not when added to patient serum previously absorbed with beef erythrocyte stroma. The test is generally simple, sensitive, and specific, but false negative are common in young children with mononucleosis.
The most common rapid slide test (MONOSPOT©) for infectious mononucleosis employs:
A) Intact beef erythrocytes
B) None of the above
C) Horse erythrocytes
D) Sheep erythrocytes
3. This is the lens power of the scanning objective lens.
A) 4X
B) 100X
C) 40X
D) 10X
4. Transpeptidase enzymes catalyze the final stage of peptidoglycan synthesis during cell wall formation.
Beta-lactam antibiotics bind with these enzymes, interfering with their function. The transpeptidase enzymes are also referred to as PBP's, or penicillin-binding proteins.
Beta-lactam antibiotics interfere with cell wall synthesis by:
A) Interfering with PBPs
B) Preventing the final stage of peptidoglycan synthesis
C) Binding with a transpeptidase
D) All of the above
5. The tube to the left is a slant of esculin medium, the tube to the right contains heart infusion broth with 6.5% sodium chloride. The bacterial species characteristically producing the positive reactions seen in each of these tubes is most likely:
A) Enterococcus faecalis
B) Streptococcus bovis
C) Staphylococcus aureus
D) Listeria monocytogenes
Solutions:
| Question # 1 Answer: B | Question # 2 Answer: C | Question # 3 Answer: A | Question # 4 Answer: D | Question # 5 Answer: C |
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