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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Macroamylasemia can be diagnosed by measuring amylase levels in the urine and serum. In patients with macroamylasemia, there will be high levels of amylase in the serum. However, macroamylasemia can look similar to acute pancreatitis, which also causes high levels of amylase in the blood. To help differentiate, we measure amylase levels in the urine. Urine levels of amylase are low in people with macroamylasemia, but high in patients with pancreatitis.
The results which would be MOST consistent with macro-amylasemia are?

A) Normal serum amylase and elevated urine amylase values
B) Increased serum and increased urine amylase values
C) Normal serum and normal urine amylase values
D) Increased serum amylase and normal to low urine amylase values

2. The beta hemoglobin chain loci are found on which chromosome?

A) Chromosome 11
B) Chromosome 16

3. Beer's law is based on the fact that absorbance is directly proportional to the concentration of a solution.
Therefore, stray light can alter the absorbance results in this type of assay.
Deviations from Beer's Law are caused by:

A) polychromatic light
B) very low concentration of absorbing material
C) very high concentrations of substance being measured in a colorimetric reaction
D) stray light

4. Measuring the turnaround times of troponin tests from the emergency department would provide valuable information. This data represents circumstances in which patient safety may be compromised if results are delayed and treatment is not started as soon as possible.
A laboratory manager wants to evaluate the timeliness of patient services in order to prevent medical errors caused by delay in treatment. Measuring the turnaround time for which of these analytes would provide the most valuable information?

A) Routine glucose tests from patients on medical floors
B) Lipid panels from the outpatient clinic
C) Troponin tests from the emergency department

5. Modified Thayer Martin agar at 35ºC with CO2 is the correct answer. This is a typical presentation of systemic gonorrhea and Neisseria gonorrhea grows best on MTM medium at 35ºC. under CO2. Systemic gonorrhea occurs in 1% of cases and results in purulent arthritis and rarely septicemia.
An 18-year-old woman woke up in the morning to find that her left knee was hot, swollen, and very painful.
She could not walk to work and a friend offered her a ride to the clinic. She had never had a swollen joint before. Physical exam revealed a tender knee joint which yielded purulent synovial fluid on aspiration.
Gram-negative intracellular diplococci were seen on Gram stain. She also had a cervical discharge that was cultured. The cause of her infection would grow best on:

A) V (human blood) agar at 35ºC with CO2
B) Modified Thayer Martin agar at 35ºC with CO2
C) LIM (Todd Hewitt Broth w/Colistin and Nalidixic Acid) broth with refrigerated temperature
D) Blood agar at 42ºC with microaerophiilic conditions

Solutions: